quantification cycle (Cq)

Definition

Depending on the real-time instrument, either threshold cycle (Ct), crossing point (Cp) or a take-off point (Top) are used to refer to the same quantification cycle value (Cq): the fractional PCR cycle at which the target is quantified in a given sample. It was proposed to use the term quantification cycle (Cq) in accordance with the data standard RDML (Real-Time PCR Data Markup Language)

References

  1. Lefever S., Hellemans J., Pattyn F., Przybylski DR., Taylor C., Geurts R. et al. 2009 RDML: structured language and reporting guidelines for real-time quantitative PCR data. In: Nucleic Acids Research. Vol. 37, (7) pp. 2065-2069. doi: 10.1093/nar/gkp056
  2. Bustin SA., Benes V., Garson JA., Hellemans J., Huggett J., Kubista M. et al. 2009 The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments. In: Clinical Chemistry. Vol. 55, (4) pp. 611-622. doi: 10.1373/clinchem.2008.112797
Proposed by: Michael Rubinigg
Properties

Label

quantification cycle (Cq)

EUPH Code

[7347]

EUPH URI

https://app.pollinatorhub.eu/vocabulary/classes/7347

Created

2024-10-08

Updated

2024-10-08
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Author: Michael Rubinigg
Definition
Author: Michael Rubinigg
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Authors: Michael Rubinigg

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English
quantification cycle

Phrase

Michael Rubinigg
Approved
English
Cq

Phrase

Michael Rubinigg
Approved